Table 2 Primers used in PCR amplification and sequencing of GNB3 C825T SNP
Primer Set | Forward Primer (5' - 3') | Reverse Primer (5'-3') | Ta (°C) |
|---|---|---|---|
Outer | GCTGCCCAGGTCTGATCCCT | CCAGTGACAAGGGACAGCAGTAAG | 60 |
Inner | TGACCCACTTGCCACCCGTGC | GCAGCAGCCAGGGCTGGC | 60 |
Sequencing | CAGTTCTTCCCCAATGGAGAGG | GGCTGGCCCTTACCCACACG | 55 |