Figure 8

Effect of TNPO3 KD on HIV-1 infection of CD4⁺ T cells. Jurkat T cells, modified with TNPO3 KD or control KD lentiviruses, were challenged with WT or CA mutant HIV-1 viral vectors, as indicated. (A) GFP reporter gene expression was checked by flow cytometry 72 hrs after HIV-1 challenge. Late RT (B), 2-LTR circles (C) and provirus (D) were assayed by qPCR 24 hrs after infection. Viruses bearing the enzymatic site mutants RT-D185K/D186L or IN-D116A IN were used as controls for de novo reverse transcription and integration, respectively. Data represent one of at least three independent experiments. Error bars represent ± SEM (n = 3).