Figure 2

The effect of TNPO3 KD on the infectivity of HIV-1 CA mutants in HeLa cells. HeLa control (ctrl) KD cells and TNPO3 KD cells were challenged with a panel of 27 HIV-1-GFP reporter vectors bearing either WT CA or the indicated CA mutants. At 72 hrs the percent GFP⁺ cells was determined by flow cytometry as an indication of infectivity. The ratio of HIV-1 infectivity in Ctrl KD vs TNPO3 KD cells is shown. Error bars represent ± SEM (n = 3). Black bars indicate mutants that were significantly less sensitive than the WT to TNPO3 KD (p < 0.01, t-test). Gray bars indicate mutants that were significantly more sensitive than the WT to TNPO3 KD (p < 0.01, t-test).