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Table 1 Quantification of 10 nm gold particle-labeled anti-p24CA in cells expressing HERC5+CS and HIV-1 Gag.

From: Human HERC5 restricts an early stage of HIV-1 assembly by a mechanism correlating with the ISGylation of Gag

Region

Observed gold count, Go

Point count, P

Expected gold count, Ge

Go/Ge

Χ2

Χ2 as %

Particles+

Plasma membrane

118

189

29

4.0

270.3

85.9

Cytoplasm+

Nucleus

80

875

135

0.6

22.5

7.1

Non-particle+

non-cell

18

335

52

0.3

22.0

7.0

TOTAL

216

1399

216

 

314.8

100.0

  1. For Χ2 = 314.8 and df = 2, P < 0.0001 (Χ2 analysis). The gold labeling distribution is significantly different from random. Only the particles/plasma membrane region (Go/Ge = 4.0, Χ2 = 85.9% of total) meets the two criteria for being preferentially labeled ((Go/Ge) > 1 and Χ2 value ≥ 10% of total).