Region | Observed gold count, Go | Point count, P | Expected gold count, Ge | Go/Ge | Χ2 | Χ2 as % |
---|
Particles+
Plasma membrane
| 118 | 189 | 29 | 4.0 | 270.3 | 85.9 |
Cytoplasm+
Nucleus
| 80 | 875 | 135 | 0.6 | 22.5 | 7.1 |
Non-particle+
non-cell
| 18 | 335 | 52 | 0.3 | 22.0 | 7.0 |
TOTAL
| 216 | 1399 | 216 | Â | 314.8 | 100.0 |
- For Χ2 = 314.8 and df = 2, P < 0.0001 (Χ2 analysis). The gold labeling distribution is significantly different from random. Only the particles/plasma membrane region (Go/Ge = 4.0, Χ2 = 85.9% of total) meets the two criteria for being preferentially labeled ((Go/Ge) > 1 and Χ2 value ≥ 10% of total).