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Figure 5 | Retrovirology

Figure 5

From: TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions

Figure 5

TRIM5α directly associates with proteasome subunit PSMC2. (A) Progressive acceptor photobleaching of YFP-PSMC2 resulted in an increase in CFP-rhTRIM5α fluorescence indicating FRET. YFP-PSMC2 or YFP empty vector and CFP-rhTRIM5α were transfected in HeLa cells at a 1:1 ratio. Over the course of 10 minutes, 50 frames were obtained for YFP and CFP channels. (B) Fluorescence intensities of CFP-rhTRIM5α and YFP-PSMC2 are recorded. EFRET is calculated as a relative increase of YFP fluorescence intensity (%) after photobleaching of the CFP FRET acceptor.

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