Figure 4
From: TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions
TRIM5α associates with PSMC2 and 20S. (A) Cells stably expressing HA-rhTRIM5α were seeded on glass coveslips and immunostained for HA using Cy5 rabbit antibody and endogenous proteasome subunit PSMC2 using Al546 mouse antibody. Following staining procedure, cells were fixed and mounted on glass slides. Cy5 channel was progressively bleached and fluorescence intensity was detected over time in Al546 and Cy5 channels. Images were analyzed using softWoRx software. (B) Cells stably expressing HA-rhTRIM5α were immunostained for HA using Cy5 rabbit antibody and endogenous 20S core particle using Al546 mouse antibody. Cy5 channel was progressively bleached and fluorescence intensity was detected over time in Al546 and Cy5 channels. Images were analyzed using softWoRx software.