Figure 3
From: TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions
Proteasomal subunits localize to TRIM5α assemblies at various frequencies. (A) HeLa cells stably expressing YFP-rhTRIM5α were fixed and immunostained with antibodies specific to the individual subunits of the proteasome. Z-stack images were collected with a DeltaVision microscope equipped with a digital camera using a 1.4-numerical aperture (NA) 100× objective lens, and were deconvolved with SoftWoRx deconvolution software. Individual channel images were superimposed to create the merged panels. (B) Deconvolved images were analyzed for subunit mean fluorescence intensity (MFI) in YFP-rhTRIM5α cytoplasmic bodies by the use of the Surface Finder function in the Imaris software (Bitplane). For each YFP-rhTRIM5α cytoplasmic body, the MFI of the subunit was determined and the data was plotted in GraphPad Prism 5® software.