Figure 1
From: TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions
Ubiquitin and PSMC2 identified as interacting proteins with huTRIM5α and TRIMCyp via yeast two-hybrid. Yeast strain bearing pSH18-34 was transformed with the bait plasmid pEG202-human TRIM5α or the bait plasmid pEG202-TRIMCyp. Sequentially, a pool of pJG4-5 prey plasmids containing a cDNA library were transfected, and screening for positive interaction proteins was performed. Screening identified two GAL4 AD fusions that activated gene transcription by lexA fusions to human and Aotus TRIM5α proteins that were sequenced and submitted for BLAST analysis.