Figure 7From: Residual HIV-1 DNA Flap-independent nuclear import of cPPT/CTS double mutant viruses does not support spreading infectionEffect of the central DNA Flap on percentage of transduced cells and mean fluorescent intensity upon increasing MOI. HeLa cells were transduced with Flap+ or Flap- HIV-derived vectors (TRIP-eGFP and HR-eGFP, respectively) [9] in 6-well plates with 0.1, 1, 10, 100 or 1000 ng/0.3 × 106 cells. The percentage of GFP-positive cells and the mean fluorescent intensity were assessed 48 h post-transduction by flow cytometry (BD Calibur).Back to article page