Figure 6
Co-depletion of β-TrCP1 and β-TrCP2 does not affect Vpu's activity to enhance virus release. 293T cells were transfected twice with siRNAs targeting either β-TrCP1 alone, β-TrCP2 alone (with one of two different siRNAs, designated β-TrCP2 1 and β-TrCP2 2 ) or combinations of siRNAs targeting β-TrCP1 and β-TrCP2 (siT1/2 1 : siRNAs for β-TrCP1+ β-TrCP2 1 , or siT1/2 2 : siRNAs for β-TrCP1+ β-TrCP2 2 ), or with a control siRNA (si Control). During the second transfection plasmids encoding HIV-1wt or HIV-1Δvpu, and HA-CD317 were added. Two days post-transfection, the yield of infectious HIV-1 in culture supernatants was quantified. The factor of difference between the infectious titers for HIV-1 wt and HIV-1Δvpu in cells expressing CD317 is depicted. The arithmetic means ± SEM of three independent experiments are shown.