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Figure 3 | Retrovirology

Figure 3

From: HIV-1 predisposed to acquiring resistance to maraviroc (MVC) and other CCR5 antagonists in vitro has an inherent, low-level ability to utilize MVC-bound CCR5 for entry

Figure 3

Profiles of MVC resistance in 293-Affinofile cells induced to express different levels of CCR5. (A) Luciferase reporter viruses pseudotyped with MVC-Res or MVC-Sens Envs were used to infect 293-Affinofile cell populations induced to express CD4 and increasing levels of CCR5 in the presence or absence of 10 μM MVC, and the percent infection was calculated relative to the levels of infection achieved in cells expressing the highest levels of CCR5 without drug, as described previously [15]. (B) The CCR5 induction levels in response to increasing concentrations of ponasterone A were quantified by qFACS as described previously [21]. (C) The inability of virus pseudotyped with MVC-Sens Env to be completely inhibited by MVC in 293-Affinofile cells expressing the highest CCR5 levels was compared to inhibition of virus pseudotyped with YU2, JRCSF, NB6-C3 or NB8-C4 Envs in these cells. The data shown are means of triplicates and are representative of 3 independent experiments. Error bars represent standard error.

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