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Figure 1 | Retrovirology

Figure 1

From: HIV-1 predisposed to acquiring resistance to maraviroc (MVC) and other CCR5 antagonists in vitro has an inherent, low-level ability to utilize MVC-bound CCR5 for entry

Figure 1

Profiles of MVC resistance in cell lines expressing different levels of CCR5. (A) U87-CD4/CCR5, JC53. TZM-bl and NP2-CD4/CCR5 cells were stained for cell-surface CCR5 expression, which was quantified by qFACS as described previously [21]. (B) Luciferase reporter viruses pseudotyped with MVC-Sens or MVC-Res Envs were used to infect the different cell lines in the presence of increasing concentrations of MVC as described in the Methods, and virus inhibition curves were generated and used to calculate the MPI as described previously [15]. The CCR5 cell surface staining data is representative of 3 independent experiments. The virus inhibition data are means of triplicates, and are derived from 3 independent experiments. Error bars represent standard error. MCF, mean cell fluorescence; MPC, molecules per cell; MPI, maximal percent inhibition.

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Retrovirology

ISSN: 1742-4690

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