Figure 6
Vaccination with attenuated SIV differentially modulates LPL and IEL CD4+CCR5+ T cell memory subsets. Following vaccination of cynomolgus macaques (n = 20) with live attenuated SIV the percentages of LPL and IEL CD4+CCR5+ TCM, TTrM-1, TTrM-2 and TEM cells from the SI was determined at days 0, 3, 7, 10, 21 and 125 post inoculation. Transient increases in the percentage of SI LPL and IEL CD4+ TEM cells with a concomitant decrease in CD4+ TTrM-2 cells was observed at day 3 (a). Increased percentages of SI LPL and IEL CD4+CCR5+ TTrM-2 and TEM cells was observed at days 3 and 7 (b). Immunostaining of small intestine LPL and IEL gated on CD3+CD4+CD95+ memory T cells from one representative animal from each time point are shown (c). In each panel the upper right hand quadrant shows CD28+CCR7+ TCM and TTrM-1 cells, the lower right quadrant CD28+CCR7- TTrM-2 cells and the lower left quadrant CD28-CCR7- TEM cells. CD4+ TTrM-1 cells are essentially CCR5+ TCM cells. Percentages shown give the proportion present in each of these subsets. CD4+CD95+CCR5+ T cells are shown in red and CD4+CD95+CCR5- T cells in green. TCM: central memory, TTrM-1: transitional memory subset-1, TTrM-2: transitional memory subset-2, TEM: effector memory, IEL: intraepithelial lymphocytes, LPL: lamina propria lymphocytes, SI: small intestine.