Figure 5
CD4+CCR5+ T CM and T EM cell dynamics in the small intestine of macaques vaccinated with attenuated SIV. Following vaccination of cynomolgus macaques (n = 20) with live attenuated SIV the percentages of CD4+CCR5+ TCM and TEM cells from the SI was determined at days 0, 3, 7, 10, 21 and 125 post inoculation. Dynamic changes in the frequency of SI CD4+ lymphocytes naïve, TCM, TEM subsets was observed following vaccination (a). Increases in the percentage of CD4+CCR5+ TCM and TEM cells was observed at days 3 and 7, reversed at days 10 and 21, then increased again at day 125 compared to naive controls (b). Immunostaining of SI lymphocytes gated on CD3+CD4+ T cells from one representative animal from each time point are shown (c). In each panel the left hand quadrant shows CD28+CD95- TN cells, the upper right quadrant CD28+CD95+ TCM cells and the lower right quadrant CD28-CD95+ TEM cells. Percentages shown give the proportion present in each of these subsets. CD4+CCR5+ T cells are shown in red and CD4+CCR5- T cells in green. TN: naive, TCM: central memory, TEM: effector memory, SI: small intestine.