Figure 8
From: Lack of adaptation to human tetherin in HIV-1 Group O and P
Residues from group M Vpu are not sufficient to confer anti-tetherin activity to group O Vpu. (A) Schematic of Vpu-FLAG proteins based on group O Vpu, with group M substitutions at positions 10, 12, 14 and 18. (B) Ability of indicated Vpu proteins to increase HIV-1 VLP release from HeLa cells, measured as previously described, p < 0.01 (**). (C) Effect of transfecting increasing amounts of plasmids O-N18A and O-3A,S12V (range 2 to 6 μg) on VLP release from HeLa cells.