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Figure 7 | Retrovirology

Figure 7

From: Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cells

Figure 7

ASP expression in infected or transfected Jurkat T cells. (A) Schematic representation of the pNL4.3ASP-Flag vector, which corresponds to a molecular proviral DNA in which ASP was tagged with the Flag epitope at its C-terminal end. Jurkat cells were infected with NL4.3ASP-Flag (B) or NL4.3ASPmut12-Flag (C) virions pseudotyped with VSVg and ASP localization was analyzed as described above. The morphology of the cell was assessed by NDIC. White bars correspond to a scale of 10 μm.

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