Figure 5From: Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cellsASP localization in membrane connection. Jurkat cells transfected with pcDNA-Flag-ASP (A-C) or pEGFP (D) were layered on glass slides (A) or seeded at high density on polylysine-covered glass slides (B-D). The localization of ASP was analyzed as described above. The morphology of the cell was assessed by NDIC. White bars correspond to a scale of 10 μm.Back to article page