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Figure 4 | Retrovirology

Figure 4

From: Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cells

Figure 4

Cellular localization of WT ASP and ASP-mut66 in transfected Jurkat T cells. Jurkat cells transfected with pcDNA-Flag-ASP (A-C) or pcDNA-Flag-ASPmut66 (D) were layered on glass slides, fixed, permeabilized, and stained with fluorescence-labelled antibodies as described in Figure. 3. The morphology of the cell was assessed by Normaski differential interference contrast (NDIC). White bars correspond to a scale of 10 μm. (E) Percentage of the total transfected cells with ASP showing an unpolarized distribution (white bar), a polarized location (grey bar), or a localization into membrane protrusion (hashed bar). A total of 206 cells from three separate experiments were scored.

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