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Figure 6 | Retrovirology

Figure 6

From: Interplay between HIV Entry and Transportin-SR2 Dependency

Figure 6

The viral entry route influences the TRN-SR2 dependency of the HIV-1 N74D CA mutant. HeLaP4 cells depleted of TRN-SR2 (siTRN-SR_2) and control cells (mock and siTRN-SR_2MM) were challenged using 3 dilutions of pseudotyped HIV-1 NL4-3 (WT) or HIV-1 NL4-3 N74D CA mutant (N74D) luciferase reporter viruses. Viruses were pseudotyped with various envelopes derived from HIV-1 (A), amphotropic MLV (B), measles virus (C), VSV (D) or Ebola virus (E). Three days post infection Fluc activity was measured and normalized to the total amount of protein in the cell lysates. Graphs show the mean values of triplicate measurements of Fluc light units per μg protein (Fluc LU/μg protein) ± SD of a representative experiment.

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