Figure 3

The HIV-1 N74D CA mutant remains partially dependent on TRN-SR2 when carrying the HIV envelope. (A) HeLaP4 cells depleted of TRN-SR2 (siTRN-SR_2) and control cells (mock and siTRN-SR_2MM) were challenged using 3 dilutions of VSV-G pseudotyped HIV-1 NL4-3 (WT) or (B) HIV-1 NL4-3 N74D CA mutant (N74D) luciferase reporter viruses. Three days post infection Fluc activity was measured and normalized to the total amount of protein in the cell lysates. Graphs show the mean values of Fluc light units per μg protein (Fluc LU/μg protein) ± SD of one representative experiment out of two performed in triplicate. (C) Same as in (A), but multiple-round viruses HIV-1 NL4-3 (WT) or (D) HIV-1 NL4-3 N74D CA mutant (N74D) carrying the HIV-1 envelope were used for infections. Infectivity was measured by β-gal activity 72 hours post infection. The arrows indicate the relative inhibition of infectivity in TRN-SR2 depleted cells compared to mismatch siRNA tranfected cells.