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Figure 1 | Retrovirology

Figure 1

From: Interplay between HIV Entry and Transportin-SR2 Dependency

Figure 1

Effect of TRN-SR2 knockdown on transduction efficiency of various retroviral vectors. (A) TRN-SR2 knockdown in HeLaP4 cells 3 days post siRNA transfection visualized by western blotting. β-tubulin was detected as a control for equal loading. (B) HeLaP4 cells were transfected with siRNAs for knockdown of TRN-SR2 (siTRN-SR_2), with a mismatched control siRNA (siTRN-SR_2MM) or were mock transfected (mock). Three days post transfection cells were transduced with different VSV-G pseudotyped retroviral vectors encoding GFP. The overall GFP fluorescence was measured by flow cytometry and is expressed as the percentage relative to the control cell values. Results represent mean values ± standard deviation (SD) of at least 3 independent experiments each performed in triplicate. In each experiment newly produced viral vectors were used.

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