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Figure 4 | Retrovirology

Figure 4

From: Subunit-specific mutational analysis of residue N348 in HIV-1 reverse transcriptase

Figure 4

RNase H cleavage activity of recombinant purified HIV-1 RT that contained mutations at residue N348. A) Schematic illustrating the location of the RNase H cleavage sites in the RNA template. B) Autoradiogram of the RNase H cleavage patterns of HIV-1 RTs containing mutations at residue 348 in both subunits of the enzyme. Experiments were carried out as described in the Methods. The reaction times were 1, 3, 5, 10, 20, 30, 45, 60 and 90 min. C) Autoradiogram of the RNase H cleavage patterns of HIV-1 RTs containing mutations at residue 348 in either the p66 or p51 subunits of the enzyme. Experiments were carried out as described in the Methods. The reaction times were 1, 3, 5, 10, 20, 30, 45, 60 and 90 min.

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