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Figure 6 | Retrovirology

Figure 6

From: G140S/Q148R and N155H mutations render HIV-2 Integrase resistant to Raltegravir whereas Y143C does not

Figure 6

In vitro RAL susceptibility of wt and mutated HIV-2 INs. Mutations were introduced in the HIV-2 N1 background by mutagenesis. (A) Representative gel of the strand transfer activity of recombinant HIV-2 IN mutants in the presence of increasing concentrations of RAL. (B) Comparison of strand transfer activity in the presence of RAL of wt (N1) (circle), E92Q (square), Y143C (triangle), E92Q/Y143C (inverted triangle) and T97A/Y143C (diamond) mutants. Strand transfer reaction was carried using a 32P-labeled oligonucleotide mimicking the preprocessed substrate and 200 nM IN, in the presence of increasing concentrations of RAL at 37°C. Activity is expressed as a % of control without drug. Experiments were performed two times.

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