Skip to main content
Figure 1 | Retrovirology

Figure 1

From: G140S/Q148R and N155H mutations render HIV-2 Integrase resistant to Raltegravir whereas Y143C does not

Figure 1

Study of catalytic properties of INs amplified from plasma of three INI-naive patients (N1, N2, N3) infected with HIV-2. Processing activity (bottom panel) and strand transfer activity (top panel) were assayed as a function of IN concentration. Both panels represent a unique gel with the top panel corresponding to a longer exposure. Full assay for catalytic activity was performed as described in Materials and methods section using a 21-mer (U5A/U5B) blunt DNA substrate (12,5 nM), with MgCl2 as a cofactor (7.5 mM). Lane 1. No IN. Lanes 2, 5, 8: 100 nM; Lanes 3, 6, 9: 200 nM; Lanes 4, 7, 10: 300 nM IN. ST: strand transfer; 3'-P: 3'-processing.

Back to article page