Figure 2
From: Orthoretroviral-like prototype foamy virus gag-pol expression is compatible with viral replication
Schematic illustration of PFV Gag-Pol expression constructs. Schematic outline of the parental proviral expression construct pczHSRV2 wt. Below enlargement of the regions of Gag-Pol ORF overlap/fusion in the individual constructs as indicated. Sequences of PFV Gag origin in dark grey and light grey boxes, of PFV Pol origin in white boxes and of HIV-1 origin in black boxes. Amino acids (aa) are given in the 1-letter code. Aa not originally encoded by either PFV or HIV-1 derived sequences but from cloning sites are in italic. CMV, cytomegalovirus promoter; R, long terminal repeat region (LTR); U5, LTR unique 5' region; U3, LTR unique 3' region; IP, internal promoter; major PFV PR cleavage sites in PFV Gag and Pol are indicated by black arrows; HIV-1 PR cleavage sites are indicated by grey arrows.