Figure 3
The ability of Nef to associate with activated PAK2 correlates with the ability to enhance T cell activation. A. MAGI R5+ cells were incubated for 6 h with 5,000 3H cpm of RT activity of VSV-G pseudotyped virus. Infections and staining were performed as in Figure 1B. Average percent of infected (β-galactosidase+) cells from triplicate infections ± SEM are shown. B and C. 200,000 3H cpm of RT activity of VSV-G pseudotyped virus were incubated overnight with 106 Jurkat cells stably expressing NFAT-Luc (B) or IL2R-Luc (C). Infected cells were then incubated with 106 α-CD3/CD28 beads for 4 h. Cells were lysed with passive lysis buffer. The lysate was freeze/thawed once and luciferase activity was assayed by luminescence. Average luciferase activity for triplicate samples ± SEM is shown.