Figure 4

Unstimulated CD8⁺ T cells from ES8 block infection by autologous and wild type virus (described in Figure 2a) as effectively as cells stimulated with autologous or wild type TW10 peptides. CD4⁺ T cells from the patient were infected via spinoculation with wild type NL43 and NL43-based viruses expressing autologous TW10 epitopes, as described in Figure 2a. Cells were then co-cultured with CD8⁺ T cells from ES8 which were either unstimulated or had been stimulated with the TVA, TIA, or AVA peptides for four days. Co-culture with infected cells was for seven days. Results shown are averages of triplicate experiments with error bars indicating standard deviation.