Table 2 Primers and probes for real-time PCR

SIV doubly spliced

Forward: 5'- AGGCTAATACATCTTCTGCATCAAAC - 3'

Reverse: 5'- GGGTCCTGTTGGGTATGAGTCTA - 3'

Probe: 5' - CCACCCTCTTATTTCC - 3'

SIV singly spliced

Forward: 5'- AGAGGCCTCCGGTTGCA-3'

Reverse: 5'- CCTTCCCCTTTCCACAATAGC-3'

Probe: 5'-ACTGTGGAAGGGACC-3'

SIV unspliced

Forward: 5'- TTGCAGCACCCACAACCA-3'

Reverse: 5'-TGATCCTGACGGCTCCCTAA-3'

Probe: 5'- CTCCACAACAAGGACA-3'

IFN-γ

Forward: 5'- GTGTGGAGACCATCAAGGAAGAC-3'

Reverse: 5'- CGACAGTTCAGCCATCACTTGGAT-3'

Probe: 5'-ACTGACTCGAATGTCCAACGCAAAGC-3'

GAPDH

Forward: 5'-GGCATCCTGGGCTACACTGA-3'

Reverse: 5'-AGGAGTGGGTGTCGCTGTTG-3'

Probe: 5'- AGGTGGTCTCCTCTGAC -3'