Representative flow cytometric analyses carried out on control uninfected and GALV-GFP-C11D8 infected MDTF cells expressing the HA-tagged GALV receptor SLC20A1 cells. The cells were stained with monoclonal antibodies against V5, HA and C11D8 epitopes as well as R-phyoerythrin conjugated goat anti-mouse isotope specific secondary antibodies. In histograms, solid purple represents control groups; blue lines represent uninfected MDTFSLC20A1-HA cells; red lines represent MDTFSLC20A1-HA cells infected with GALV-GFP-C11D8 viruses. The relative amounts of cell surface detected V5-tagged GALV RBD (A), HA-tagged SLC20A1 (B) GALV envelope tagged with C11D8 epitope (C) and V5-tagged A-MLV RBD (D) are shown on the x-axis. In these experiments, we employed MDTF or CHOK1 cells as negative controls (data not shown). The experiment was performed for three independent times with similar results.