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Figure 1 | Retrovirology

Figure 1

From: The receptors for gibbon ape leukemia virus and amphotropic murine leukemia virus are not downregulated in productively infected cells

Figure 1

A schematic representation of the viruses used in this study. A-MLV-GFP and GALV-GFP are replication-competent MoMLV in which the MLV envelope (env) gene has been replaced with either A-MLV [14] or GALV env [14, 15]. Both viruses contain an IRES-GFP cassette between the env gene and 3'LTR. In addition, GALV-GFP also contains an insertion of TCC just upstream of the splice acceptor (SA) resulting in a virus with enhanced infection and replication properties [15]. GALV-GFP-C11D8 is identical to GALV-GFP except that the C11D8 epitope tag (QVMTITPPQAMGPNLVLP) that derives from the amino acid terminus of the FeLV-B proline rich region (PRR) was introduced into the GALV PRR [37]. The relative position of PRR within SU and transmembrane (TM ) subunits of GALV envelope protein is shown. GALV-Gag tomato red was generated by replacing GFP of GALV-GFP with Gag fused in frame to fluorescent tomato red gene in the GALV-GFP plasmid. The retroviral vector plasmid, pRT43.2 βgal contains a CMV immediate early enhancer/promoter in the 5' LTR as well as a β-galactosidase reporter gene.

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