Figure 4
Susceptibility of HIV-infected MDMs to recognition by CTLs of another specificity. MDMs prepared from an HIV-negative donor (HLA-A*24:02+) infected with the indicated viruses were used as target cells for cytolysis by an HLA-A24-restricted CTL clone specific for the Nef epitope (Nef138-147: RYPLTFGWCF). The frequency of HIV-infected cells among the target cells, as determined by intracellular p24 Ag expression, was 41.4, 48.3, 44.5, 40.8, 40.0, and 45.0% for wt, 85F, 75T, TF, M20A, and P82A variants, respectively. CTL activity toward uninfected cells was deducted from the data as background. An additional experiment showed similar results.