SOSIP.R6-IZ-CD40L induces DC maturation. A. Monocyte-derived iDC were cultured for 48h in the presence of SOSIP.R6-IZ, SOSIP.R6-IZ-CD40L or control stimuli. The expression of the maturation marker CD83 was monitored by FACS. B. CD83 was measured on DC stimulated for 48 h with a combination of TNF-α/IL-1β and SOSIP.R6-IZ, SOSIP.R6-IZ-CD40L or control stimuli. Gray lines represent the isotype-matched control. Cells were double-stained for CD11c and CD83 and CD83 Histograms of cells gated on CD11c are shown (> 25,000 events).