Chlorhexidine Reduces Expression of HIV-1 Rev. Cells were untreated or treated for 4-5 h with TG009 or chlorhexidine, then expression of the endogenous HIV-1 provirus was induce by addition of doxycycline. Twenty-four hours later, cells were harvested and cell extracts fractionated on SDS-PAGE gels. Resultant blots generated were probed with anti-Rev, anti-Tat or anti-tubulin antibodies to assess the effect of drugs on viral protein expression. Results shown are representative of >3 independent trials.