Effect of Chlorhexidine on HIV-1 RNA Accumulation and Splicing. Cells were treated for 4-5 h with TG003, TG009 or chlorhexidine (5 μM), then doxycycline was added to induce expression of the endogenous HIV-1 provirus. Twenty-four hours later, cells were harvested and total RNA extracted. (A) Abundance of US, SS, and MS viral RNAs was determined by qRT-PCR as outlined in "Materials & Methods". Shown are the average of >5 independent analyses. (B) To examine the effect of drug treatment on viral RNA splicing, radioactive RT-PCR was performed on MS viral RNAs and products fractionated on 8 M urea-PAGE gels followed by exposure to phosphor screens to detect the different splice products. On the left is a representative gel of the pattern observed and on the right, a summary of the relative abundance of each splice product over multiple assays (n > 6). Asterisks denote values determined to be significantly different from control at a p value < 0.05.