Figure 5
From: Differential effect of CLK SR Kinases on HIV-1 gene expression: potential novel targets for therapy
TG003 and Chlorhexidine Alter the Effect of CLK Kinases on SRSF2 (SC35) Subcellular Distribution. Cells were transfected with GFP-CLK expression vectors. Forty-eight hours post-transfection, cells were treated with (A) 10 μM TG003 or (B) 10 μM chlorhexidine for 4-5 h then fixed and processed for immunofluorescence localization of SRSF2. Cells were stained with anti-SRSF2 antibody followed by Texas Red conjugated donkey anti-mouse antibody and nuclei stained with DAPI. Shown are representative samples of the localization patterns of the CLKs and SRSF2 observed. Magnification 630x.