Effect of CLK Overexpression on SRSF2 (SC35) Subcellular Distribution. Cells were transfected with GFP-CLK expression vectors, incubated for forty-eight hours post-transfection, and then fixed and processed for immunofluorescence localization of SRSF2. Cells were stained with anti-SRSF2 antibody followed by Texas Red-conjugated donkey anti-mouse antibody and nuclei stained with DAPI. Shown are representative samples of the localization patterns of the CLKs and SRSF2 observed from > 5 experiments. Magnification 630x.