Figure 7
NC WT phenotype is dominant over NC mutants with no correlation between infectivity and premature reverse transcription. 293T cells were cotransfected with the indicated ratios of NCWT or NCmutant proviral plasmids. The percentage of NCWT in each transfection is shown in blue. The resulting viruses were harvested and infectious titer in TZM-bl cells was determined (red). The resulting titers were corrected for input virus and are expressed as a percentage of WT titer. The amount of intravirion DNA (R-U5) was also quantified in each sample (green), and these values are expressed as a percentage of intravirion DNA present for each respective NCmutant virus (so that 100% mutant is defined as 100% intravirion DNA). The values are the average of 2 separate experiments and the error bars indicate the standard deviations.