Phenotypic identification of thymic DC. (A) DC were gated based on CD123, CD11clo or CD11chi expression. (B) A major plasmacytoid (pDC; HLA-DRintCD123hiCD11c-; 77.1%); a smaller myeloid (HLA-DRint/hiCD123-CD11clo; 20.4%); and a minor myeloid-related M-DC8+ (HLA-DRint/hiCD123-CD11chiM-DC8+; 2.5%) DC population were identified. Expression of myeloid antigens (CD1c and CD14), adhesion and co-stimulatory molecules (CD11c and CD11b), HIV-1 receptors (CD4, CXCR4 and CCR5), maturation markers (CD83 and CD86), C-type lectin receptors (DC-SIGN, DEC-205 and MR), CD123, HLA-DR and M-DC8 was determined. Mean fluorescence intensity (MFI) > 2000 (+++), MFI 500-2000 (++), MFI 60-500 (+) or absent (-). * ≥ 50% of population positive (representative data from 2-3 separate experiments).