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Figure 4 | Retrovirology

Figure 4

From: HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells

Figure 4

Determination of apoptotic cell percentage by a flow cytometry procedure. Sub-confluent MSCs treated with HIV-1 strains (5 ng/ml p24) and recombinant gp120 (1 μg/ml) were assayed (panel A) with propidium iodide staining after cell fixation at different times (days 1, 3, and 7). Panel B reports the apoptosis induction when hiHIV-1 strains or gp120 were used. Panel C represents the apoptotic cell percentages obtained when CD4 blockade by p5p treatment was performed. Statistical significance was determined using Student's t test with *p < 0.05.

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