Figure 5
From: Trafficking of some old world primate TRIM5α proteins through the nucleus
Localization of TRIM5α rh N-terminal deletion mutants in leptomycin B-treated cells. Cf2Th cells stably expressing wild-type TRIM5αrh or the indicated deletion mutant were treated with 5 ng/ml of LMB or DMSO for two hours. Treated cells were stained using anti-HA antibodies conjugated to FITC. TRIM5αrh domains are depicted for each variant, and the numbers of the amino acid residues at the boundaries of the different domains are shown (A). L1 represents the Linker 1 region. The TRIM5αrh∆93 protein bodies are located in the cellular nucleus (B). Cf2Th cells expressing TRIM5αrh∆93 were stained using using anti-HA antibodies conjugated to FITC (green) and propidium iodide for nuclear staining (red). A representative image is shown.