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Table 6 Comparison of expression trends identified by microarray or RT-qPCR in independent RNA preparations

From: Tat RNA silencing suppressor activity contributes to perturbation of lymphocyte miRNA by HIV-1

Expression Trend in Microarrays Expression Relative to Mock Measured by qPCR a
  HIV-1 RSS ΔVV
Upregulated    
hsa-miR-198 8.3 ± 1.0 8.3 ± 2.2 9.5 ± 0.3
hsa-miR-214 8.6 ± 4.5 15.3 ± 5.4 12.7 ± 5.7
Downregulated    
hsa-miR-29a 0.8 ± 0.1 0.6 ± 0.1 1.0 ± 0.3
hsa-miR-128 1.1 ± 0.4 1.0 ± 0.2 0.9 ± 0.1
  1. a Change in expression for indicated miRNAs was measured by qRT-PCR using Taqman probes in independent RNA preparations of HIV-1, RSS, ΔVV, and mock infected cells. Values for quantitative RT-PCR are derived from at least three replicate experiments, and expressed relative to mock. Relative expression differences were calculated using the ΔΔCT method with efficiency correction and RNU48 as the internal control.