Tat RNA silencing suppressor activity contributes to perturbation of lymphocyte miRNA by HIV-1

Retrovirology

Table 1 Percentage of CEMx174 infected cells at time of RNA harvest

	Percentage of Virus Infected Cells ^a
Experiment	Mock	HIV-1	RSS	ΔVV
Replicate 1	0	90	83	80
Replicate 2	0	95	87	90

^a CEMx174 cells were infected by co-culture and the progression of infection was monitored by FACS of intracellular Gag. Values indicate the percentage of Gag⁺ cells at time of harvest. Total cellular RNA was prepared in Trizol, integrity verified by bioanalyzer and processed for the miRNA microarrays.

ISSN: 1742-4690