Figure 2
From: A Functional Role for ADAM10 in Human Immunodeficiency Virus Type-1 Replication
ADAM10 silencing persists for one week following siRNA transfection. Assessment of ADAM10 expression following RNAi by (A) flow cytometry in TZM-bl cells 48 hours after transfection, (B) Western blot in primary human macrophages 48 hours after transfection, and real time PCR in (C) U373-MAGI-CCR5 cells and (D) primary human macrophages at various times after siRNA transfection. Relative levels of ADAM10 mRNA expression in siRNA transfectants were normalized to GAPDH expression (n = 4).