Figure 2

HIV-1 transfer into J-ps20^high cells is dependent on virus fusion and leads to higher levels of productive infection. (A) J-Ps20^high and J-ps20^inter target cells stained with DDAO SE vital dye were seeded at 1 × 10⁵ cells per well of a 24 well plate in the presence or the absence of 5 μg/ml of T-20 for 1 hour prior to co-cultured with 18% Jwt ps20^inter NL4-3-infected donor cells at a T:D ratio 1:0.2. Mean percentage of Gag⁺ J-ps20^inter vs. J-ps20^high target cells 4 hours post co-culture is shown. Data represent mean of three replicate assays. (B) The dye-labelled J-Ps20^high and J-ps20^inter target cells were seeded at 1 × 10⁵ cells in the presence or the absence of 5 μM of RT-inhibitors (AZT+Lamimidine) for 1 hour prior to co-culture with 25% NL4-3-infected donor cells at a T:D ratio of (B) 1:0.2 or (C) 1:1. The percentage of Gag⁺ J-ps20^inter vs. J-ps20^high target cells +/- RT inhibitors were assessed at 4, 24 and 72 hours post co-culture. Data represent the mean of three replicate assays. Asterisks denotes statistically significant data as calculated using a paired t-test (*P ≤0.05; **P ≤0.01).