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Figure 1 | Retrovirology

Figure 1

From: WFDC1 expression identifies memory CD4 T- lymphocytes rendered vulnerable to cell-cell HIV-1 transfer by promoting intercellular adhesive junctions

Figure 1

Jurkat CD4+ T cells stably transduced to express full-length human ps20 are rendered more susceptible to T-T HIV-1 transfer. (A) Representative dot plots of dye labelled target cells co-cultured with uninfected (transfer control) or infected donor cells at 4 and 24 hours post co-culture. (B) Mean percentage of Gag+ J-ps20inter vs. J-ps20high target cells at 4 hours post co-culture with 36% NL4-3 Jwt-ps20inter donor cells at T:D ratio of 1:1 and 1:0.2. Data represent mean of three replicate assays. (C) Mean percentage of Gag+ J-ps20inter vs. J-ps20high target cells at 24 hours post co-culture with 36% NL4-3 infected Jwt-ps20inter donor cells at T:D ratio of 1:1 and 1:0.2. Data represent mean of three replicate assays. (D) Mean percentage of Gag+ J-ps20inter vs. J-ps20high target cells at 4 hours post co-culture with YU2 infected Jwt-ps20inter donor cells at T:D ratio of 1:0.2. Data represent mean of three replicate assays. (E) Target cells co-cultured with 40% YU2 infected donor cells were sorted for dye-positive single cells based on both FSC height vs. width followed by SSC height vs. width, on a BD FACS Aria II cell sorter. DNA extracted from these sorted singlet cells was subject to qDNA PCR for HIV-1 LTR. The level of HIV-1 LTR in J-ps20inter vs. J-ps20high target cells is shown relative to β-actin expression and normalized against DNA isolated from 8E5 cells. Asterisks denotes statistically significant data as calculated using an unpaired t-test (*P ≤0.05; **P ≤0.01; ***P ≤0.001)

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