Sedimentation equilibrium analysis of NYAD-203 on CA. Data were collected at 20,000 rpm, 25°C and pH 7.3 using the Beckman XL-A/XL-I Analytical ultracentrifugation. The CA concentration was fixed at 30 μM. (A, B & C) The residual difference between the fitted curve and the experimental data and (A
) Plot of Absorbance at 280 nm vs. centrifugal radius in the absence (control) or presence of 30 μM and 90 μM of NYAD-203, respectively. The open circles represent the experimental data and the solid lines are the best fit using an ideal single-species model.