Nef enhances the expression levels of IFIT-1 mRNA in HIV-1-infected iDCs. The levels of IFIT-1 mRNA in iDCs mock infected or infected with WT NL(AD8) or NL(AD8)ΔNef were quantified using real-time RT-PCR. The IFIT-1 expression levels were normalized by the relative GAPDH expression levels. The results of mock-infected controls were assigned as 1 and the fold enhancement of IFIT-1 expression in HIV-1 infected iDCs is shown. WT, wild-type NL(AD8); ΔNef, NL(AD8)ΔNef. One representative experiment out of three is presented.