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Figure 8 | Retrovirology

Figure 8

From: Tetherin does not significantly restrict dendritic cell-mediated HIV-1 transmission and its expression is upregulated by newly synthesized HIV-1 Nef

Figure 8

HIV-1 replication in iDCs upregulates tetherin in a Nef-dependent manner. (A) Cell lysates from iDCs infected with WT NL(AD8), NL(AD8)ΔNef with (+) or without (-) AZT or mock infected were evaluated at 5 dpi for the expression of HIV-1 Gag (p55 and p24), tetherin and GAPDH by immunoblotting. (B) Flow cytometry analysis of surface tetherin expression in infected DCs at 5 dpi. M, Mock infection; WT, wild-type NL(AD8); ΔNef, NL(AD8)ΔNef. One representative experiment out of three is shown.

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