Pro-inflammatory stimuli upregulate tetherin expression in DCs. Tetherin expression on iDCs, mDC-LPS and mDC-IFNα from two different donors was assessed by (A) flow cytometry and (B) immunoblotting. (C) TNF-α treatment of DCs modestly upregulates tetherin expression compared with mDC-LPS and mDC-IFNα. Tetherin expression was detected by immunoblotting. (D) HEK293T and HeLa cells were used as negative and positive controls, respectively. Numbers shown in flow cytometry plots are % positive (top) and the geometric mean values of fluorescence intensity (bottom) for each histogram.