IFNα induces DC maturation but does not alter the expression of HIV-1 receptors. iDC, mDC-LPS and mDC-IFNα were stained for cell surface expression of CD4, CCR5, DC-SIGN and CD86. On each histogram, the filled peaks are the controls of isotype or secondary antibody alone and the black peaks represent the staining of specific markers. Top and bottom numbers shown in plots are % positive and the geometric mean values of fluorescence intensity, respectively. Results shown are from DCs from a single donor representative of two independent experiments on DCs from different donors.